Hello there

I have a few basic bioMEMS queries. A device of mine has an array of
cantilevers with one side of each coated with biomolecules (antibodies).

*How do I coat different cantilevers with different antibodies? ( I donot
want antibodies for cantilever A to get onto cantilever B)

*what is a micro/nano pipette? (Do I need it for the above qn?)

*I am doing a test of antigens in blood. So what I have is a chamber with
my cantilever(s) and serum floating about. Say the blood does have a
particular qty of antigens and it does get stuck to the antibodies
specific to it on the cantilever. Thus there is a deflection which I
measure and hence I know how many molecules got stuck to the cantilever
(can someone also elaborate on how i do this). Now how do I relate the
concentration of the antigens in the serum to that on my cantilever? Is it
probabilistic? Or do I have to aim a fixed qty of serum only on the
cantilever so that every (?) molecule gets stuck????

Plz help me out.

Thanks a million

Samir

Samir Kagadkar
Deptt of Electrical Engg
Indian Institute of Technology Bombay