Greetings all-

  I'm looking for a software package to track microspheres in a channel.

  Specifically, I am visualizing 10um diameter microspheres in fluid
flowing through straight 100um-200um wide microchannels.  I've recorded
videos of the fluid flow shown by the moving microspheres and I'm trying
to track the microspheres to determine the fluid flow in the channel.
The problem is that some microspheres are stuck to the channel which
makes interpretation difficult.
  I've tried DiaTrack software which identifies the particles which move
as well as the particles that are stuck to the channel.  The problem is
that the software gets confused by the fixed particles and interprets
them as moving particles resulting in particle trajectories that falsely
appear to move against the flow.
  Is there a standard software package to use for 2D flow visualization?
 Any suggestions for this specific problem?  Any and all advice is
welcome.

thanks in advance,
Michael